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Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Human Frizzled-10 (C-6His) (Cat#orb2992704) at 2 μg/ml (100 μl/well) can bind Anti-Human Frizzled-10 mab (Human IgG1). The ED50 of Anti-Human Frizzled-10 mab (Human IgG1) is 10.53 ng/ml. (Regularly tested) |
| Tag | C-6His |
| Molecular Weight | Predicted: 16.9 KDa. Observed: 18-28 KDa, reducing conditions |
| Expression Region | Ile21-Gly161 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxins | ＜ 1 EU/μg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Frizzled-10; Fz-10; Fzd10; Fz10; FZD10
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Recombinant Human Frizzled-10 Protein (orb2992704)
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