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Description
Research Area
Immunology & Inflammation
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Human LY75 (C-Fc) (Cat#orb2992705) at 5 μg/ml (100 μl/well) can bind Anti-Human CD205 mAb (Human IgG1)*. *:Biotinylated by NHS-biotin prior to testing. The ED50 of Anti-Human CD205 mAb (Human IgG1) is 4.282 ng/ml. (Regularly tested). |
| Tag | C-Fc |
| Expression Region | Ser28-Asp1666 |
| Endotoxins | ＜ 1 EU/μg as determined by LAL test. |
| MW | Predicted: 215.7 KDa. Observed: 175-260 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Lymphocyte antigen 75; Ly-75; C-type lectin domain family 13 member B; CD205; LY75; CLEC13B
Similar Products
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Recombinant Human CD205/LY75 Protein, N-His [orb2969886]
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>90% as determined by SDS-PAGE.
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Human CD205 Protein, His Tag [orb1173821]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 189.6 kDa after removal of the signal peptide.
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10 μg, 100 μg, 50 μg
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Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide