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Human RSPO3
SKU: orb2993001
Description
Images & Validation
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Key Properties
−| Expression System | Human cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | Cell Based Assay: Human breast cancer organoids were cultured with Wnt3a(Cat#orb3002512),Noggin (Cat#orb3002494),R-spondin 3 (Cat#orb2993001),KGF(Cat#orb3002467), FGF-10(Cat#orb3002478),EGF (Cat#orb3002493)and NRG1-beta 1(Cat#753). DAPI (blue), Ki67(red) and ER(green). |
| Tag | C-6His |
| Molecular Weight | Predicted: 14.7 kDa. Observed: 15-25 kDa, reducing conditions |
| Expression Region | Gln22-Val146 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 90% as determined by SEC-HPLC.(QC verified) |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−R-spondin-3; RSPO3; Protein with TSP type-1 repeat; Roof plate-specific spondin-3; Thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2; CRISTIN1
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Human RSPO3 (22-146) Protein, hFc Tag [orb1173794]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 38.6 kDa after removal of the signal peptide. The apparent molecular mass of RSPO3(22-146)-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
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Human RSPO3 (orb2993001)
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