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Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Anti-Human TF Antibody (TIS_bio, Research Grade)(Cat#orb2992713) at 1μg/ml (100 μl/well) can bind Recombinant Human TF (C-Fc)(Cat#orb2992804)*. *: Biotinylated by NHS-biotin prior to testing.The EC50 of Recombinant Human TF (C-Fc)(Cat#orb2992804) is 2.71 ng/ml.(Regularly tested). |
| Tag | C-Fc |
| Expression Region | Ser33-Glu251 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| MW | Predicted: 51.6 KDa. Observed: 66-77 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tissue Factor; TF; Coagulation Factor III; Thromboplastin; CD142; F3
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Quick Database Links
UniProt
UniProt Details
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide