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Description
Research Area
Immunology & Inflammation
Images & Validation
−
Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Recombinant Human Transferrin (Cat#orb2994488) on SA Biosensor, can bind Recombinant Human TFRC (N-6His) (Cat#orb2994094) with an affinity constant of 99.1 nM as determined in BLI assay. (Regularly tested) |
| Tag | N-6His |
| Expression Region | Leu101-Phe760 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 74.9 KDa. Observed: 80-90 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, 5% Mannitol, 0.01% Tween80, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Transferrin receptor protein 1; TR; TfR; Trfr; T9; p90
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Protein Handling and Storage Guide
Protein Handling Guide
Recombinant Human TfR Protein (orb2994094)
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