Recombinant Human VEGF165 Protein

SKU: orb2995236

Description

Recombinant Human Vascular Endothelial Growth Factor A is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human
Biological Activity	ELISA: Immobilized Recombinant Human VEGF165(Cat#orb2995236) at 2μg/ml (100 μl/well) can bind Human VEGF-A Antibody (Bevacizumab)(Human IgG1)(Cat#orb2992936). The ED50 of Human VEGF-A Antibody (Bevacizumab)(Human IgG1)(Cat#orb2992936) is 2.20 ng/ml. (Regularly tested) Immobilized Human VEGF165 (Cat#orb2995236) at 10μg/ml (100 μl/well) can bind Human VEGFR1-Fc (Cat#orb2994454). The ED50 of Recombinant Human VEGFR1-Fc (Cat#orb2994454) is 36.4 ng/ml. (Regularly tested). BLI: Loaded Brolucizumab on AMC Biosensor, can bind Recombinant Human VEGF165 (Cat#orb2995236) with an affinity constant of 22.2 pM as determined in BLI assay. (Regularly tested) Loaded Human VEGF-A Antibody (Bevacizumab) (Cat#orb2992936) on Pro-A Biosensor, can bind Recombinant Human VEGF165 (Cat#orb2995236) with an affinity constant of 7.12 pM as determined in BLI assay. (Regularly tested) Loaded Human VEGFR1-Fc(Cat#orb2994454) on Protein A Biosensor, can bind Human VEGF165(Cat#orb2995236) with an affinity constant of 0.29 nM as determined in BLI assay. (Regularly tested). Cell Based Assay: Measured by its ability to induce calcineurin- NFAT signaling in 293-VEGF Res cells(Cat#Xorb3002411). The ED50 for this effect is 1.56 ng/mL.(Regularly tested)
Tag	Tag free
Molecular Weight	Predicted: 19.1 KDa. Observed: 18-24 KDa, reducing conditions
Expression Region	Ala27-Arg191
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxins	< 0.01 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of 20mM Citrate, 8% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 4.0.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Vascular Endothelial Growth Factor Isoform 165; VEGF165

Human VEGF165 Protein, hFc Tag [orb2321384]

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 45.3 kDa after removal of the signal peptide. The apparent molecular mass of VEGF165-hFc is approximately 35-55 kDa due to glycosylation.
Source:	Mammalian

Sizes Available: 50 μg, 100 μg, 10 μg

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UniProt

P15692

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