Recombinant Human Wnt3a surrogate (Active)

SKU: orb2986910

Description

This Recombinant Human Wnt3a surrogate (Active) spans the amino acid sequence from region 1-306aa. Purity: Greater than 90% as determined by SDS-PAGE.

Images & Validation

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Application Notes	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	Mammalian cell
Biological Origin	Homo sapiens (Human)
Biological Activity	Measured by its ability to induce Topflash reporter activity in HEK293T cells. The ED50 for this effect is 17.76-26.50 ng/mL.
Tag	C-terminal hFc1-tagged
Molecular Weight	58.5 kDa
Expression Region	1-306aa
Protein Length	Full Length
Purity	Greater than 90% as determined by SDS-PAGE.
Endotoxins	Less than 1.0 EU/ug as determined by LAL method.

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Form/Appearance	Lyophilized powder
Buffer/Preservatives	Lyophilized from a 0.2 μm sterile filtered 25 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.2
Disclaimer	For research use only

Alternative Names

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