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Recombinant Mouse IL-17A Protein
SKU: orb2994008
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | BLI: Loaded Biotinylated Human IL-17RA -His-Avi(Cat#orb2993686) on SA Biosensor, can bind Mouse IL-17A-His(Cat#orb2994008) with an affinity constant of 12.6 nM as determined in BLI assay.. Cell Based Assay: Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells.The ED50 for this effect is 0.25-1.25 ng/ml. (QC verified) |
| Tag | C-6His |
| Molecular Weight | Predicted: 16.2 KDa. Observed: 17-26 KDa, reducing conditions |
| Expression Region | Thr22-Ala158 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-17A; IL-17; IL-17A; Cytotoxic T-Lymphocyte-Associated Antigen 8; CTLA-8; IL17A; CTLA8; IL17
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Recombinant Mouse IL-17A Protein (orb2994008)
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