Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep)

SKU: orb1674352

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Description

This Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep) spans the amino acid sequence from region 1-601aa. Purity: Greater than 85% as determined by SDS-PAGE.

Images & Validation

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Application Notes	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	Yeast
Biological Origin	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Tag	N-terminal GST-tagged
Molecular Weight	92.3 kDa
Expression Region	1-601aa
Protein Length	Full Length
Protein Sequence	AVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCNAPGCDVTDVTQLYLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDFNAIATCDWTNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDRELHLSWEVGKPRPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVGDYFVLTSHTVMPLSAPTLVPQEHYVRITGLYPTLNISDEFSSNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARARVECFDKFKVNSTLEQYVFCTVNALPETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQLPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLKAHKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFISPYNSQNAVASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNVAITRAKVGILCIMSDRDLYDKLQFTSLEIPRRNVATLQ
Purity	Greater than 85% as determined by SDS-PAGE.

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Form/Appearance	Liquid or Lyophilized powder
Buffer/Preservatives	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Disclaimer	For research use only

Alternative Names

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(pp1ab)(ORF1ab polyprotein)(Leader protein)(Non-structural protein 1)(nsp1)(nsp2)(p65 homolog)(Non-structural protein 3)(nsp3)(PL2-PRO)(Papain-like proteinase)(PL-PRO)(nsp4)(3CL-PRO)(3CLp)(Main protease)(Mpro)(Non-structural protein 5)(nsp5)(SARS coronavirus main proteinase)(nsp6)(nsp7)(nsp8)(nsp9)(nsp10)(Growth factor-like peptide)(GFL)(Pol)(RdRp)(Non-structural protein 12)(nsp12)(Hel)(Non-structural protein 13)(nsp13)(ExoN)(Guanine-N7 methyltransferase)(Non-structural protein 14)(nsp14)(NendoU)(Non-structural protein 15)(nsp15)(Non-structural protein 16)(nsp16)

Quality Guarantee

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Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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UniProt

P0DTD1

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