RecombinantIFN-γ,Human

SKU: orb1494843

Description

Human Interferon gamma (hIFN-γ) is amacrophage‐activating factor and the lone member of Interferon type II.The active form of IFN-γ is an antiparallel dimer that interacts with the receptor IFN-γR1 and sets off IFN-γ/JAK/STAT pathway. IFN-γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity. While IFN-γ–induced inflammatory cascade summons a variety of immune‐related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN-γ is also implicated in resistance to NK cell and CTL responses and in immune escape in a variety of cancers.Recombinant human Interferon gamma (rhIFN-γ) produced in E.coli is a non‐glycosylated polypeptide chain of 144 amino acids. A fully biologically active molecule, rhIFN-γ has a molecular mass of 17 kDa analyzed by reducing SDS‐PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O or PBS at 100 μg/ml.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50<0.05ng/ml, measured by cytotoxicity assay using HT‐29 cells.
Molecular Weight	17kDa, observed by reducing SDS‐PAGE.
Protein Sequence	MQDPYVKEA ENLKKYFNAG HSDVADNGTL FLGILKNWKE ESDRKIMQSQ IVSFYFKLFK NFKDDQSIQK SVETIKEDMN VKFFNSNKKK RDDFEKLTNY SVTDLNVQRK AIHELIQVMA ELSPAAKTGK RKRSQMLFRG RRASQ
Purification	> 95% as analyzed by reducing SDS‐PAGE.
Purity	> 95% as analyzed by reducing SDS‐PAGE.
Endotoxins	< 1 EU/μg, determined by LAL

Storage & Handling

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Storage	Lyophilized recombinant human Interferon gamma (rhIFN‐γ) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIFN-γ should be stable up to 1 week at 4°C or up to 2 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI,IFN-gamma, IFNγ

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