Retinoic acid

SKU: orb1309934

Description

Tretinoin, a vitamin A metabolite, is a potent natural agonist of retinoic acid receptors (RARα/β/γ) with an IC50 of 14 nM. It is widely used in research to study cellular differentiation, proliferation, and tumorigenesis in both in vitro and in vivo models of oncology and dermatology.

Research Area

Cell Biology, Metabolism Research, Molecular Biology

Images & Validation

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Key Properties

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CAS Number	302-79-4
MW	300.44
Purity	99.60%
Formula	C₂₀H₂₈O₂
SMILES	CC(/C=C/C1=C(C)CCCC1(C)C)=C\C=C\C(C)=C\C(O)=O
Target	PPAR,Endogenous Metabolite,Autophagy,Retinoid Receptor
Solubility	Ethanol:6 mg/mL (19.97 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5.6 mg/mL (18.64 mM);DMSO:95 mg/mL (316.2 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

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Target IC50	RARβ:14 nM\|RARγ:14 nM\|PPARβ/δ:17 nM (Kd)\|PPARα:103 nM (Kd)\|PPARγ:178 nM (Kd)\|RARα:14 nM
In Vivo	In studies evaluatin the impact on glutathione levels and catalase activity, Tretinoin increased both metrics in a time- and dose-dependent manner, offering Protective and mitigating effects against H2O2 cytotoxicity in human renal mesangial cells. Treatment with Tretinoin resulted in elevated mRNA levels of catalase and γ-glutamylcysteine synthetase (the catalytic subunit responsible fo the rate-limiting step in reduced glutathione synthesis) in cultured mesangial cells. Additionally, Tretinoin upregulated matrix metalloproteinase-8/13 in human keloid-derived fibroblasts. Read more...
In Vitro	Tretinoin prevents skin atrophy induced by corticosteroids in hairless mice. When co-administered with miquimod in guinea pigs, tretinoin induces tattoo fading and moderate pigment clearance histopathologically. Applications of tretinoin on incisions in the skin of 45 CD-1 mice increase fibroblast differentiation and reduce collagen production. In aged Male Fischer 344 rats treated with tretinoin, renal cortex protein content is 30% lower compared to controls, potentially due to suppressed expression of tumor necrosis factor-β1 and osteopontin. Read more...
Cell Research	Retinoic acid is dissolved in DMSO and stored, and then diluted with appropriate medium before us. P19 cell are induced to undergo neuronal differentiation according to established procedures. Briefly, cells are cultured on 1% agarose-coated 10 cm dishes at × 0 5 cells mL in α-minimal essential medium supplemented with 10% FBS. Differentiation is induced by addition of Retinoic acid (1 μM) and medium containing Retinoic acid replaced 2 days later. On day 4, cell aggregates are collected by centrifugation, separated to Single cells by trypsin/EDTA treatment, replated onto poly-L-lysine-coated plates, and cultured in α-minimal essential medium supplemented with 10% FBS. On day 6, medium is replaced with neurobasal medium containing B27 supplement and 2 mM GlutaMAX. Medium is replaced every 2 days for an additional wee. Read more...

Storage & Handling

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Storage	keep away from direct sunlight,store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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all-trans-Retinoic acid, Autophagy, ATRA, inhibit, Inhibitor, Endogenous Metabolite, EndogenousMetabolite, Vitamin A acid, RAR/RXR, RAR, PPAR, Peroxisome proliferator-activated receptors, Retinoic acid receptors, Retinoic acid, Retinoid Receptor, Retinoid X receptors, RetinoidReceptor, Tretinoin

Quality Guarantee

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Compound Identifiers

PubChem CID

444795 ↗

Key Properties

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No computed properties available.

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Retinoic acid (orb1309934)

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Applications:	FC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Gallus, Porcine, Rat
Reactivity:	Human, Mouse
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	IF, IHC-Fr, IHC-P
Predicted Reactivity:	Human, Mouse, Rabbit
Reactivity:	Human, Mouse, Rabbit, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	IF, IHC-Fr, IHC-P
Predicted Reactivity:	Bovine, Porcine
Reactivity:	Human, Mouse, Rabbit, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, FC, IHC, WB
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	FC, IF, IHC-Fr, IHC-P
Predicted Reactivity:	Bovine, Equine, Gallus, Porcine, Rabbit
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Retinoic acid

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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