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RFC2 Rabbit Polyclonal Antibody

SKU: orb158278

Description

RFC2 Rabbit Polyclonal Antibody

Research Area

Epigenetics and Nuclear Signaling

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P, WB
Dilution RangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityMouse
Predicted ReactivityBovine, Canine, Equine, Human, Porcine, Rat, Sheep

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human RFC2 (281-354/354aa)
TargetRFC2
Molecular Weight39 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

RFC40; 2610008M13Rik; 40kDa; Recc2; RFC2_HUMAN; RFC2; Activator 1 40 kDa subunit (A1 40 kDa subunit); Activator 1 subunit 2; Replication factor C 40 kDa subunit (RF-C 40 kDa subunit | RFC40); RFC2_MOUSE; RFC2_RAT; Activator 1 subunit C2;

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RFC2 Rabbit Polyclonal Antibody

Sample: Bone (Mouse) Lysate at 40 ug, Primary: Anti-RFC2 (orb158278) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kD, Observed band size: 39 kD.

RFC2 Rabbit Polyclonal Antibody

Tissue/Cell: mouse intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-RFC2 Polyclonal Antibody, Unconjugated (orb158278) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

RFC2 Rabbit Polyclonal Antibody (orb158278)

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Available Sizes

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50 μl
¥ 3,250.00
100 μl
¥ 5,070.00
200 μl
¥ 6,110.00
Free Secondary Antibody (20 ul)0/0

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