RIG-I Antibody (C-term)

SKU: orb1932676

This product can be custom made

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Infectious Disease & Virology

Images & Validation

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Item 1 of 3

Tested Applications	WB
Dilution Range	WB - 1:2000
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	This RIG-I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 894-925 amino acids from the C-terminal region of human RIG-I. Antigen Region: 894-925 aa.
Target	RIGI (HGNC:19102)
Molecular Weight	106600 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Probable ATP-dependent RNA helicase DDX58, DEAD box protein 58, RIG-I-like receptor 1, RLR-1, Retinoic acid-inducible gene 1 protein, RIG-1, Retinoic acid-inducible gene I protein, RIG-I, DDX58

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western blot analysis of lysates from THP-1 cell line, untreated or treated with LPS, 1ug/ml, using DDX58 C-term K909 (upper) or Beta-actin (lower).

24-hour post infection immunoblots of whole cell lysates from primary murine microglia cells (2x106) untreated (0) or exposed to vesicular stomatitis virus at a range of viral particle/cell ratios.

All lanes: Anti-DDX58 C-term K909 at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HUVEC whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: mouse spleen lysate. Lane 5: mouse thymus lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 107 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

RIGI (HGNC:19102)

UniProt

O95786

RefSeq (Protein):NP_055129.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_055129.2

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Protocol Information

Western Blot (IB, immunoblot)

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