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RNF2 Antibody
SKU: orb1257433
Featured
Description
Images & Validation
−Item 1 of 6
| Tested Applications | ChIP, IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RNF2 (NP_009143.1). |
| Target | RNF2 |
| Molecular Weight | Observed: 38kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−RNF2, BAP1, DING, BAP-1, HIPI3, RING2, RING1B
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Western blot analysis of extracts of various cell lines, using RNF2 antibody (orb1257433) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using RNF2 antibody (orb1257433) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RNF2 antibody (orb1257433) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RNF2 antibody (orb1257433) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug RNF2 antibody (orb1257433). Western blot was performed from the immunoprecipitate using RNF2 antibody (orb1257433) at a dilution of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RNF2 antibody (orb1257433) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Quick Database Links
Gene Symbol
RNF2
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
ChIP
Chromatin Immunoprecipitation
RNF2 Antibody (orb1257433)
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