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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 7
| Tested Applications | ICC, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 , IP: 1:100-1:500 |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary. |
| Target | ROCK1 |
| Molecular Weight | 158 |
| Purity | Purified by antigen-affinity chromatography. |
| Purification | Purified by antigen-affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Buffer/Preservatives | PBS, 1% rAlbumin, 20% Glycerol, 0.025% ProClin 300. |
| Concentration | 0.78 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Rho associated coiled-coil containing protein kinase 1 , P160ROCK , ROCK-I
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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Immunofluorescence analysis of HeLa cells using ROCK1 antibody
Immunohistochemical staining of human breast carcinoma using ROCK1 antibody
Immunoprecipitation analysis of 293T whole cell using ROCK1 antibody
Western blot analysis of Various whole cell using ROCK1 antibody
Western blot analysis of NIH-3T3 whole cell(Lane 1), C2Cl2 whole cell(Lane 2) using ROCK1 antibody
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Western blot analysis of Various whole cell using Thymidylate synthase antibody
Quick Database Links
Gene Symbol
ROCK1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ICC
Immunocytochemistry
IP
Immunoprecipitation
ROCK1 Rabbit Polyclonal Antibody (orb556838)
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