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RPL5 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human RPL5 (NP_000960.2). |
| Target | RPL5 |
| Molecular Weight | Observed: 37kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of extracts of various cell lines, using RPL5 antibody (orb1258356) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded mouse liver using RPL5 antibody (orb1258356) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using RPL5 antibody (orb1258356) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of MCF-7 cells using RPL5 antibody (orb1258356). Blue: DAPI for nuclear staining.
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RPL5 Antibody (orb1258356)
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