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RPL5 Antibody

SKU: orb1258356
FeaturedFeatured Product

Description

RPL5 Antibody

Research Area

Cancer Biology, Neuroscience

Images & Validation

Tested ApplicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
Application Notes
WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human RPL5 (NP_000960.2).
TargetRPL5
Molecular WeightObserved: 37kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

L5, MSTP03PPP1R135, uL18, 60S ribosomal protein L5, large ribosomal subunit protein uL18, protein phosphatase 1, regulatory subunit 135

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RPL5 Antibody

Western blot analysis of extracts of various cell lines, using RPL5 antibody (orb1258356) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

RPL5 Antibody

Immunohistochemistry of paraffin-embedded mouse liver using RPL5 antibody (orb1258356) at dilution of 1:200 (40x lens).

RPL5 Antibody

Immunohistochemistry of paraffin-embedded mouse kidney using RPL5 antibody (orb1258356) at dilution of 1:200 (40x lens).

RPL5 Antibody

Immunofluorescence analysis of MCF-7 cells using RPL5 antibody (orb1258356). Blue: DAPI for nuclear staining.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol

RPL5 Antibody (orb1258356)

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Available Sizes

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100 μl
¥ 10,790.00
DispatchUsually dispatched within 5-10 working days
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