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RPS6KA3 Antibody
SKU: orb1254434
Featured
Description
Research Area
Cell Biology, Immunology & Inflammation, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 68-327 of human RPS6KA3 (NP_004577.1). |
| Target | RPS6KA3 |
| Molecular Weight | Observed: 84kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Ribosomal protein S6 kinase alpha-3, S6K-alpha-3, 90 kDa ribosomal protein S6 kinase 3, p90-RSK 3, p90RSK3, Insulin-stimulated protein kinase 1, ISPK-1, MAP kinase-activated protein kinase 1b, MAPK-activated protein kinase 1b, MAPKAP kinase 1b, MAPKAPK-1b, Ribosomal S6 kinase 2, RSK-2, pp90RSK2, RPS6KA3, ISPK1, MAPKAPK1B, RSK2
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Western blot analysis of extracts of mouse lung, using RPS6KA3 antibody (orb1254434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using RPS6KA3 antibody (orb1254434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RPS6KA3 antibody (orb1254434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using RPS6KA3 antibody (orb1254434) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using RPS6KA3 antibody (orb1254434). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
RPS6KA3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
RPS6KA3 Antibody (orb1254434)
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