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Recombinant FSP1 Antibody / S100A4

SKU: orb639913

Description

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsFACS, IF, IHC-P, WB
Dilution RangeFlow cytometry: 1-2ug/10^6 cells in 0.1ml,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
ReactivityHuman, Mouse
Application Notes
Optimal dilution of the recombinant FSP1 antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityRecombinant
IsotypeMouse IgG1, kappa
Clone No.rS100A4/1481
ImmunogenA recombinant human partial protein (amino acids 1-200) was used as the immunogen for the recombinant FSP1 antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC-P
Immunohistochemistry Paraffin
View Protocol
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
View Protocol
IF
Immunofluorescence
View Protocol

Available Sizes

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20 μg
$ 370.00
100 μg
$ 750.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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