SAR-20347

SKU: orb1303059

Description

SAR-20347 is a potent small molecule inhibitor targeting the TYK2, JAK1, JAK2, and JAK3 kinases with respective IC50 values of 0.6, 23, 26, and 41 nM. This compound is a valuable research tool for investigating JAK/STAT signaling pathways in both cellular and animal models of autoimmune and inflammatory diseases.

Research Area

Cardiovascular Research, Epigenetics & Chromatin, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Key Properties

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CAS Number	1450881-55-6
MW	444.84
Purity	99.77% (May vary between batches)
Formula	C₂₁H₁₈ClFN₄O₄
SMILES	NC(=O)c1nc(oc1Nc1ccc(cc1)C(=O)N1CCOCC1)-c1c(F)cccc1Cl
Target	Tyrosine Kinases,JAK
Solubility	DMSO:55 mg/mL (123.64 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.5 mM)

Bioactivity

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Target IC50	JAK2:26nM\|JAK1:23nM\|TYK2:0.6 nM\|JAK3:41 nM
In Vivo	Compared to vehicle-treated animals, SAR-20347 (60 mg/kg) inhibits the production of IFN-γ in the serum by 91%, demonstrating that SAR-20347 can inhibit TYK2 signaling in vivo. SAR-20347 markedly reduces IL-17 production as measured by average signal intensity, consistent with the gene expression analysis. Read more...
In Vitro	SAR-20347 effectively inhibits IL-12-mediated STAT4 phosphorylation (TYK2-dependent, IC50: 126 nM) when NK-92 cells are stimulated with IL-12. In the culture media, cells without IL-12 have no measurable IFN-γ, while cells incubated with IL-12 and SAR-20347 demonstrate dose-dependent inhibition of IFN-γ production. SAR-20347 (maximum effect: 5 μM) dose-dependently inhibits the production of secreted embryonic alkaline phosphatase (SEAP). Read more...
Cell Research	Cells are plated in a 96-well v-bottom plate in starvation medium, incubated with SAR-20347 (0.5% DMSO) for 20 minutes at 37°C, 5% CO2, and stimulated with individual cytokines. P-STAT levels are measured in duplicate using MSD plates following the manufacturer's instructions (MSD). Read more...
Animal Research	Female 7 to 9-week old C57BL/6 mice are used. Mice are administered vehicle or 50 mg/kg SAR-20347 by oral gavage 30 minutes prior to application of 62.5 mg 5% imiquimod cream or control cream. Another dose of vehicle or 50 mg/kg SAR-20347 is given 5.5 hours following the first dose. This treatment is repeated for 5 days and on day 3 and 4, animals are injected with 100 uL saline to prevent dehydration. On the 6th day, the animals are euthanized and photographs are taken. Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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SAR20347, SAR-20347, SAR 20347, TyrosineKinases, Tyrosine Kinases, TYK2, JAK, JAK1, Janus kinase, inhibit, JAK2, JAK3, Inhibitor

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Compound Identifiers

PubChem CID

71727668 ↗

Key Properties

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No computed properties available.

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SAR-20347 (orb1303059)

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Purity:	>98% (HPLC)
CAS Number:	1450881-55-6
MW:	444.847
Formula:	C₂₁H₁₈ClFN₄O₄

SAR-20347

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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