Non-structural protein 2 Antibody

SKU: orb1239074

Description

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC
Reactivity	Virus

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-SARS-CoV-2 (COVID-19) NSP2 antibody (orb1239074) was raised against a peptide corresponding to 15 amino acids near the center of SARS-CoV-2 (COVID-19) NSP2 protein. The immunogen is located within 50-100 amino acids of the SARS-CoV-2 (COVID-19) NSP2 protein.
Target	Non-structural protein 2
Purification	SARS-CoV-2 (COVID-19) NSP2 Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	SARS-CoV-2 (COVID-19) NSP2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Disclaimer	For research use only

Alternative Names

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NSP2, p65 homolog

NSE2/MMS21 Rabbit Polyclonal Antibody [orb2898]

Applications:	ELISA, ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Equine, Human, Mouse, Rabbit, Rat, Sheep
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 100 μl, 200 μl, 50 μl

Influenza A Nuclear export protein Rabbit Polyclonal Antibody (Biotin) [orb450456]
Applications:
ELISA, ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:
Virus
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Biotin
Sizes Available: 100 μl

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Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP2 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) NSP2 antibody (orb1239074, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP2 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.

ELISA Validation with SARS-CoV-2 (COVID-19) NSP2 Protein. Antibodies: SARS-CoV-2 (COVID-19) NSP2 Antibody, orb1239074 A direct ELISA was performed using SARS-CoV-2 NSP2 recombinant protein (orb1231565) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP2 antibody as the capture antibody Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.

Quick Database Links

Gene Symbol

Non-structural protein 2

UniProt

P0DTC1

RefSeq:YP_009742609.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	YP_009742609.1

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Protocol Information

IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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