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Non-structural protein 8 Antibody
SKU: orb1239071
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC |
|---|---|
| Reactivity | Virus |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-SARS-CoV-2 (COVID-19) NSP8 antibody (orb1239071) was raised against a peptide corresponding to 14 amino acids near the center of SARS-CoV-2 (COVID-19) NSP8 protein. The immunogen is located within 60-110 amino acids of SARS-CoV-2 (COVID-19) NSP8. |
| Target | Non-structural protein 8 |
| Purification | SARS-CoV-2 (COVID-19) NSP8 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | SARS-CoV-2 (COVID-19) NSP8 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−NSP8
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Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP8 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) NSP8 antibody (orb1239071, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP8 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA Validation with SARS-CoV-2 (COVID-19) NSP8 Protein. Antibodies: SARS-CoV-2 (COVID-19) NSP8 Antibody, orb1239071. A direct ELISA was performed using SARS-CoV-2 NSP8 recombinant protein (orb1231575) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP8 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | YP_009742615.1 |
|---|
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Protocol Information
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Non-structural protein 8 Antibody (orb1239071)
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