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ORF8 Antibody
SKU: orb1239057
Featured
Description
Images & Validation
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| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Virus |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-SARS-CoV-2 (COVID-19) ORF8 antibody (orb1239057) was raised against a peptide corresponding to 13 amino acids near the central domain of SARS-CoV-2 (COVID-19) ORF8 protein. The immunogen is located within 30-80 amino acids of the SARS-CoV-2 (COVID-19) ORF8 protein. |
| Target | ORF8 |
| Molecular Weight | 14kD |
| Purification | SARS-CoV-2 (COVID-19) ORF8 Antibody is affinity chromatography purified via peptide column. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | SARS-CoV-2 (COVID-19) ORF8 antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−ORF8 protein, ns8, ORF8, Non-structural protein 8
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Western Blot Validation with SARS-CoV-2 (COVID-19) ORF8 Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF8 recombinant protein (orb1231554). Antibodies: SARS-CoV-2 (COVID-19) ORF8, orb1239057, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL and. Lane 2: 2 µg/mL.
ELISA Validation. Antibodies: SARS-CoV-2 (COVID-19) ORF8 Antibody, orb1239057. A direct ELISA was performed using SARS-CoV-2 ORF8 immunogen peptide (orb1231667) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF8 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ORF8 Antibody (orb1239057)
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