SARS-CoV-2 (COVID-19) NSP8 Protein

Name: SARS-CoV-2 (COVID-19) NSP8 Protein
Brand: Biorbyt
SKU: orb640164

SKU: orb640164

Description

NSP8, forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. To ultimately combat the emerging COVID-19 pandemic, it is desired to develop an effective and safe vaccine against this highly contagious disease caused by the SARS-CoV-2 coronavirus. By investigating the entire proteome of SARS-CoV-2, six proteins, including the S protein and five non-structural proteins (nsp3, 3CL-pro, and nsp8-10) were predicted to be adhesins, which are crucial to the viral adhering and host invasion. The S, nsp3, and nsp8 proteins were also predicted by Vaxign-ML to induce high protective antigenicity.

Research Area

Infectious Disease & Virology

Images & Validation

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Application Notes	This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 23.9 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE).

Key Properties

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Source	SARS-CoV-2 NSP8, His Tag (orb640164) is expressed from E. coli cells. It contains AA Ala 1 - Gln 198 (Accession # YP_009725304.1).
Expression System	E. coli
Target	NSP8
Reactivity	Virus
Tag	N-10×His
Expression Region	Ala 1 - Gln 198
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated
MW	23.9 kDa
Purity	95%

Storage & Handling

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Storage	-70℃
Form/Appearance	Liquid
Buffer/Preservatives	20 mM Tris, 250 mM NaCl, pH8.5
Expiration Date	6 months from date of receipt.
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

Alternative Names

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NSP8,nsp8,Non-structural protein 8

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Quick Database Links

Gene Symbol

NSP8

UniProt

YP_009725304.1

RefSeq:YP_009725304.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	YP_009725304.1

Protocol Information

Protein Handling and Storage Guide

Protein Handling Guide

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MW:	22.7KD
Source:	E. coli

Purity:	Amylose Resin (0.95)
MW:	Mono-Isotopic Mass: 66, 078.48 daltons Average Mass: 66, 120.00 daltons
Source:	bacteria

Purity:	GSH-Agarose (0.95)
MW:	Mono-Isotopic Mass: 48, 673.74 daltons Average Mass: 48, 705.25 daltons
Source:	bacteria

Purity:	>85% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining.
MW:	24.18kDa
Source:	E. coli

Purity:	≥ 90% as determined by SDS-PAGE
Source:	Recombinant SARS-CoV-2 NSP8 was expressed from E.coli expression system and the target gene encoding Ala1-Gln198 is expressed with N-His Tag