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ORF3a Antibody
SKU: orb1239063
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC |
|---|---|
| Reactivity | Virus |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-SARS-CoV-2 (COVID-19) ORF3a antibody (orb1239063) was raised against a peptide corresponding to 15 amino acids near the amino-terminus of SARS-CoV-2 (COVID-19) ORF3a protein. The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF3a protein. |
| Target | ORF3a |
| Purification | SARS-CoV-2 (COVID-19) ORF3a Antibody is affinity chromatography purified via peptide column. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | SARS-CoV-2 (COVID-19) ORF3a antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−ORF3a protein, Accessory protein 3a, Protein 3a, Protein U274, Protein X1, ORF3a
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Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF3a in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) ORF3a antibody (orb1239063, 0.2 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF3a protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA Validation. Antibodies: SARS-CoV-2 (COVID-19) ORF3a Antibody, orb1239063. A direct ELISA was performed using SARS-CoV-2 ORF3a immunogen peptide (orb1231674) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF3a antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.
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Protocol Information
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ORF3a Antibody (orb1239063)
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