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ORF3b Antibody
SKU: orb1239062
Featured
Description
Research Area
Infectious Disease & Virology
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Reactivity | Virus |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-SARS-CoV-2 (COVID-19) ORF3b antibody (orb1239062) was raised against a peptide corresponding to 16 amino acids near the central domain of SARS-CoV-2 (COVID-19) ORF3b protein. The immunogen is located within 30-80 amino acids of the SARS-CoV-2 (COVID-19) ORF3b protein. |
| Target | ORF3b |
| Molecular Weight | 17kD |
| Purification | SARS-CoV-2 (COVID-19) ORF3b Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | SARS-CoV-2 (COVID-19) ORF3b antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−ORF3b protein, Acessary protein 3b, ns3b, Non-structural protein 3b, ORF3b
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Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF3b in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) ORF3b antibody (orb1239062, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF3b protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA Validation with SARS-CoV-2 (COVID-19) ORF3b Protein. Antibodies: SARS-CoV-2 (COVID-19) ORF3b Antibody, orb1239062. A direct ELISA was performed using SARS-CoV-2 ORF3b recombinant protein (orb1231625) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF3b antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.
Western Blot Validation with SARS-CoV-2 (COVID-19) ORF3b Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF3b recombinant protein (orb1231625). Antibodies: SARS-CoV-2 (COVID-19) ORF3b, orb1239062, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 µg/mL and. Lane 2: 0.5 µg/mL.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ORF3b Antibody (orb1239062)
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