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SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein
SKU: orb1231589
Featured
Description
Research Area
Infectious Disease & Virology
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, WB |
|---|
Key Properties
−| Source | HEK293 cells |
|---|---|
| Tag | His tag at C-terminus |
| Molecular Weight | 76.2 kD |
| Protein Sequence | Val16-Arg685 |
| Purity | < 95% |
Storage & Handling
−| Storage | The protein is stable in a liquid state at -70°C for 12 months. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS |
| Concentration | 1 mg/mL |
| Expiration Date | 6 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−SARS-CoV-2 S, coronavirus spike Protein, cov spike Protein, ncov RBD Protein, ncov s1 Protein, ncov s2 Protein, novel coronavirus spike Protein, RBD Protein, S1 Protein, S2 Protein, Spike RBD Protein
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ELISA Validation of SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein, Coating Antigen: SARS-CoV-2 (COVID-19) Spike S1 recombinant protein (orb1231589) from 0.3 ng to 1000 ng. Capture Antibody: SARS-CoV-2 (COVID-19) Spike S1 antibody, orb1239981 (1 μg/mL). Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution.
SDS-PAGE Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein. Loading: 1 μg SARS-CoV-2 (COVID-19) Spike S1 recombinant protein, orb1231589. Observed: 125kD
Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein. Coating: SARS-CoV-2 (COVID-19) Spike S1 recombinant protein (orb1231589) from 3 ng to 1000 ng. Capture: ACE2 recombinant protein, orb1231604 (0.5 μg/mL). Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike S1 recombinant protein, orb1231589. Antibodies: SARS-CoV-2 (COVID-19) Spike S1, orb1239981, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL and Lane 2: 2 μg/mL.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein (orb1231589)
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