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SARS-CoV-2 Spike Trimer Protein, His Tag (KP.3) (MALS verified)

SKU: orb3158198
This product can be custom made

Description

SARS-CoV-2 Spike Trimer Protein, His Tag (KP.3) is expressed from human 293 cells (HEK293). It contains AA Val 16 -Pro 1213 (Accession # QHD43416.1 (T19I, R21T, LPP24-26Del, A27S, S50L, H69del, V70del, V127F, G142D, Y144del, F157S, R158G, N211del, L212I, V213G, L216F, H245N, A264D, I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445H, G446S, N450D, L452W, L455S, F456L, N460K, S477N, T478K, N481K, V483del, E484K, F486P, Q493E, Q498R, N501Y, Y505H, E554K, A570V, D614G, P621S, H655Y, N679K, P681R, N764K, D796Y, S939F, Q954H, N969K, V1104L, P1143L, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Research Area

Infectious Disease & Virology; Immunology & Inflammation

Images & Validation

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SARS-CoV-2 Spike Trimer Protein, His Tag (KP.3) (MALS verified)
Application Notes
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 137.7 kDa. The protein migrates as 150-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression SystemHEK293
TargetSpike protein
ReactivityVirus
TagC-10×His
Expression RegionVal 16 - Pro 1213
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW137.7 kDa
Purity90%

Storage & Handling

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Storage-20°C
Form/AppearancePowder
Buffer/Preservatives0.1 M Sodium citrate, pH 5.5
Expiration Date6 months from date of receipt.
DisclaimerFor research use only
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

Gene Symbol
Spike protein
RefSeq:Q498, F456, 26, L216, D405, LPP24, K356, N450, N969, R685, F817, I332, A892, K417, G142, N460, Y144, F486, T19, R408, Q954, V213, K986, N481, V70, V483, N501, A570, D796, R683, G446, V127, QHD43416.1, N440, H655, A264, E554, T478, N679, S373, A942, S375, L455, N764, V445, V987, S477, V1104, H245, A27, R21, Q493, A899, R158, R403, S939, Y505, G339, P681, S371, N211, F157, D614, H69, E484, L212, P621, T376, S50, P1143, L452

NCBI Reference Sequences

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Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqQ498, F456, 26, L216, D405, LPP24, K356, N450, N969, R685, F817, I332, A892, K417, G142, N460, Y144, F486, T19, R408, Q954, V213, K986, N481, V70, V483, N501, A570, D796, R683, G446, V127, QHD43416.1, N440, H655, A264, E554, T478, N679, S373, A942, S375, L455, N764, V445, V987, S477, V1104, H245, A27, R21, Q493, A899, R158, R403, S939, Y505, G339, P681, S371, N211, F157, D614, H69, E484, L212, P621, T376, S50, P1143, L452

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
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Email

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