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SARS-CoV-2 Trimeric Spike
Description
Research Area
Images & Validation
−Key Properties
−| Source | CHO-based cell line (expressed by QMCF Technology) |
|---|---|
| Molecular Weight | 417.33 kDa; Protein contains amino acids 14-1211, plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS). Also, two stabilizing prolines (PP) have been added to the S2 domain. |
| Purification | Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter. |
Storage & Handling
−| Storage | Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles. Shipping on dry ice. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4 |
| Concentration | 1 mg/ml |
| Expiration Date | 6 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
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HPLC analytical SEC for final product.
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Protocol Information
SARS-CoV-2 Trimeric Spike (orb1108524)
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