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SARS-CoV-2 Trimeric Spike

SKU: orb1108524

Description

Protein contains amino acids 14-1211, plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS). Also, two stabilizing prolines (PP) have been added to the S2 domain.

Research Area

Infectious Disease & Virology

Images & Validation

Key Properties

SourceCHO-based cell line (expressed by QMCF Technology)
PurificationMetal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.
MW417.33 kDa; Protein contains amino acids 14-1211, plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS). Also, two stabilizing prolines (PP) have been added to the S2 domain.

Storage & Handling

StorageStore at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles. Shipping on dry ice.
Buffer/PreservativesPBS pH 7.4
Concentration

1 mg/ml

Expiration Date6 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

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UniProt Details

No UniProt data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
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Available Sizes

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10 μg
$ 510.00
50 μg
$ 990.00
100 μg
$ 1,710.00
DispatchUsually dispatched within 5-10 working days
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