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SARS S Protein, His Tag

SKU: orb750333

Description

SARS S protein (R667A, K968P, V969P), His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P)). The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.

Images & Validation

Application Notes
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 136.3 kDa. The protein migrates as 190-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

Expression SystemHEK293
TargetSpike protein
ReactivityVirus
TagC-10×His
Molecular Weight136.3 kDa
Expression RegionSer 14 - Pro 1195
Purity95%
Endotoxins1.0 EU per μg
ConjugationUnconjugated

Storage & Handling

Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/AppearancePowder
Buffer/Preservatives50 mM Tris, 150 mM NaCl, 0.2 M Sodium citrate, pH 5.5
DisclaimerFor research use only

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SARS S Protein, His Tag (orb750333)

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100 μg
¥ 13,520.00
1 mg
¥ 92,040.00
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