SARS S Protein, His Tag

SKU: orb750333

Description

SARS S protein (R667A, K968P, V969P), His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P)). The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.

Research Area

Infectious Disease & Virology

Images & Validation

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Application Notes	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 136.3 kDa. The protein migrates as 190-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293
Target	Spike protein
Reactivity	Virus
Tag	C-10×His
Molecular Weight	136.3 kDa
Expression Region	Ser 14 - Pro 1195
Purity	95%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C
Form/Appearance	Powder
Buffer/Preservatives	50 mM Tris, 150 mM NaCl, 0.2 M Sodium citrate, pH 5.5
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

SARS-CoV-2 (2019-nCoV) S protein RBD, His Tag [orb689397]

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 37-38 kDa after removal of the signal peptide.
Source:	Mammalian

Sizes Available: 100 μg, 10 μg, 50 μg

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Quick Database Links

Gene Symbol

Spike protein

UniProt

P13567

RefSeq:V969, AAP13567.1, R667, K968

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	V969, AAP13567.1, R667, K968

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