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SKAP2 Antibody

SKU: orb1246979

Description

SKAP2 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in lysates of cell line Jurkat (calculated MW of 41.2kDa according to NP_003921.2). In transfected HEK293 transiently expressing SKAP2 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. The calculated Recommended concentration: 0.5-2µg/mL. An additional band of unknown identity was also consistently observed in Jurkat at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-GLVPKAYIMEMYDI
TargetSKAP2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

SKAP2, SCAP2, PRAP, src family associated phosphoprotein 2, MGC10411, MGC33304, RA70, SAPS, SCAP2, SKAP-HOM, SKAP55R, Fyn-associated phosphoprotein SKAP55 homologue, Pyk2/RAFTK-associated protein, src family associated phosphoprotein 2, src kinase-associated phosphoprotein of 55-related protein, src-associated adaptor protein

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SKAP2 Antibody

orb1246979 (0.5 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SKAP2 Antibody

HEK293 overexpressing SKAP2 and probed with orb1246979 (mock transfection in first lane).

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

SKAP2 Antibody (orb1246979)

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Available Sizes

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0.1 mg
¥ 11,960.00
DispatchUsually dispatched within 5-10 working days
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