SCRIPT RT-qPCR ProbesMaster

SCRIPT RT-qPCR ProbesMaster is designed for quantitative real-time analyses of RNA templates using Dual Labeled Fluorescent Probes. The ready-to-use mix is based on a genetically engineered reverse transcriptase with enhanced thermal stability providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments.\nThe 2x conc. mix contains all reagents required for RT-qPCR (except template, primers and the dual labeled fluorescent probe) to ensure fast and easy preparation with a minimum of pipetting steps. The premium quality enzymes and the optimized reaction buffer containing ultrapure dNTPs ensure superior real time PCR results.\nSCRIPT RT-qPCR ProbesMaster is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR, the Hot Start Taq polymerase synthesizes DNA molecules that are complementary to the cDNA, thus generating a double-stranded DNA template. In the following cycle rounds, the DNA polymerase amplifies this double-stranded DNA template exponentially.\nIn one-step RT-qPCR, all components for reverse transcription and PCR are combined in one tube so that both reactions take place one after the other without opening the tube. This offers enormous convenience when analyzing targets from multiple RNA samples and minimizes the risk of contamination.\nThe master mix already contains an optimized amount of RNase inhibitor to prevent a decrease in sensitivity due to the degradation of RNA by RNase contamination when using small amounts of templete material.\nThe mix can also be used in combination with ROX reference dye (#PCR-351) in PCR instruments that are compatible with the evaluation of the ROX signal.\nSCRIPT RT-qPCR ProbesMaster is also available as SCRIPT RT-qPCR ProbesMaster Lyophilisate, #PCR-159 in a freeze-dried (lyophilized) version that is pre-aliquoted in splittable 96-well plates, stable at ambient temperature, significantly reducing the risk of contamination, turnaround time and overall cost.