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SDS-PAGE Protein Loading Buffer 5X (Reducing)

SKU: orb90545

Description

SDS-PAGE Protein Loading Buffer is a ready-to-use 5X solution. It contains 10% SDS, 500Mm DTT, 50% Glycerol, 500mM Tris-HCL and 0.05% bromophenol blue dye. It can be used for SDS-PAGE protein loading of conventional proteins. It is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. It is sufficient to prepare 15 mL protein samples. The whole sample preparation process can be finished within 5 minutes.

Images & Validation

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SDS-PAGE Protein Loading Buffer 5X (Reducing)
Application Notes
Assay Protocol 1. Equilibrate SDS-PAGE Protein Loading Buffer 5X to room temperature or thaw Loading Buffer in a water bath no higher than 30°C. 2. Mix one volume of SDS-PAGE Protein Loading Buffer 5X with four volume of protein sample (i.e. add 4mL protein sample into 1 mL Loading Buffer). 3. Boil sample for 3-5 min. Note: If semitransparent viscous substance remains after boiling, boil sample for another 5-10 min or add 1X SDS-PAGE Protein Loading Buffer which is diluted from 5X SDS-PAGE Protein Loading Buffer to the sample and then boil for another 3-5 min. 4. Allow sample to cool to room temperature. 5. Load sample into the wells of the SDS-PAGE gel and begin electrophoresis. 6. Stop electrophoresis when bromophenol blue dye front reaches to the bottom of the gel. General Notes 1. SDS-PAGE Protein Loading Buffer 5X contains DTT which has a slightly irritating odor. 2. SDS-PAGE Protein Loading Buffer 5X contains DTT which has a slightly irritating odor. 3. Please wear the lab coat and disposable gloves and operate in a laboratory hood.

Key Properties

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Storage & Handling

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StorageUpon receipt store at -20°C. SDS-PAGE Protein Loading Buffer 5X (Reducing) is stable for one year. Product is shipped on ice.
Form/AppearanceLiquid
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protocol Information

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Li-Juan Nie 1 2, Zhe Cheng 1, Yi-Xian He 1, Qian-Hua Yan 1, Yao-Huan Sun 1, Xin-Yi Yang 1, Jie Tian 1, Peng-Fei Zhu 1, Jiang-Yi Yu 1, Hui-Ping Zhou 3, Xi-Qiao Zhou Role of duodenal mucosal resurfacing in controlling diabetes in rats World J Diabetes, (2025)

PubMed: 40093272

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Available Sizes

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3 ml
$ 60.00
20 ml
$ 100.00
DispatchUsually dispatched within 2-4 weeks
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