SEMA4F Rabbit Polyclonal Antibody

SKU: orb579783

Description

Rabbit polyclonal antibody to SEMA4F

Images & Validation

−

Item 1 of 4

Tested Applications	WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat

Related Conjugates & Formulations

−

Biotin

FITC

HRP

Key Properties

−

Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the n terminal region of human SEMA4F
Target	SEMA4F
Molecular Weight	67kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

−

S4F, SEMAM, SEMAW, M-SEMA, PRO2353, m-Sema-M

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

human cell line A431, Primary antibody dilution and incubation time: 1:600, 4 degree overnight. Secondary antibody used and dilution and incubation time: 1:3000, RT 2 hours.

Lanes: 1. A431 cell lysate + control siRNA 2. A431 cell lysate + SEMA4F siRNA, Primary Antibody dilution: 1:600, Secondary Antibody: Donkey Anti-rabbit HRP, Secondary Antibody dilution: 1:3000, Gene Name: SEMA4F.

Lanes: 1. Untransfected MIA-PACA2 cell lysate, 2. Untransfected MIA-PACA2 cell lysate, 3. Untransfected MIA-PACA2 cell lysate, 4. hSEMA4F transfected MIA-PACA2 cell lysate, 5. Untransfected MIA-PACA2 cell lysate, 6. hSEMA4F transfected MIA-PACA2 cell lysate, Primary Antibody dilution: 1:600, Secondary Antibody: Donkey Anti-rabbit HRP, Secondary Antibody dilution: 1:3000, Gene Name: SEMA4F.

WB Suggested Anti-SEMA4F Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: Transfected 293T.

Quick Database Links

Gene Symbol

SEMA4F

UniProt

O95754

RefSeq:AAH18361

UniProt Details

−

No UniProt data available

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	AAH18361

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

Find More Protocols