Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein Protein

SKU: orb1478135

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Description

This Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein Protein spans the amino acid sequence from region 319-541aa((K417N,E484K,N501Y). Purity: Greater than 90% as determined by SDS-PAGE.

Research Area

Microbiology

Images & Validation

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Item 1 of 1

Application Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	Mammalian cell
Biological Origin	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Tag	C-terminal 10xHis-tagged
Molecular Weight	27.9 kDa
Expression Region	319-541aa(K417N,E484K,N501Y)
Protein Length	Partial
Protein Sequence	RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGNIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF
Purity	Greater than 90% as determined by SDS-PAGE.
Endotoxins	Less than 1.0 EU/μg as determined by LAL method.

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Form/Appearance	Lyophilized powder
Buffer/Preservatives	Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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(S glycoprotein)(E2)(Peplomer protein)

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Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein Protein

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Quick Database Links

UniProt

P0DTC2

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No UniProt data available

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