SFRS15 Rabbit Polyclonal Antibody

SKU: orb317846

This product can be custom made

Featured

Description

SFRS15 Rabbit Polyclonal Antibody

Images & Validation

−

Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Porcine, Rabbit, Rat, Sheep

Related Conjugates & Formulations

−

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human SFRS15 (501-600/1147aa)
Target	SCAF4
Molecular Weight	126 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

CTD-binding SR-like protein RA4; DKFZp434E098; FLJ23364; KIAA1172; Pre mRNA splicing SR protein rA4; SCAF4; SFR15_HUMAN; Splicing factor arginine/serine rich 15; Splicing factor serine alanine 15; SR like CTD associated factor 4; SR-related and CTD-associated factor 4; SRA4.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SFRS15) Polyclonal Antibody, Unconjugated (orb317846) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-SFRS15 (orb317846) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 126 kD, Observed band size: 126 kD.

Sample: Thymus (Mouse) Lysate at 40 ug, K562 (Human) Cell Lysate at 30 ug, HL60 (Human) Cell Lysate at 30 ug, SIHA (Human) Cell Lysate at 30 ug, Primary: Anti-SFRS15 (orb317846) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 126 kD, Observed band size: 126 kD.

Quick Database Links

Gene Symbol

SCAF4

UniProt

O95104

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

View Protocol

Immunofluorescence

View Protocol

Find More Protocols