SFTPA1 + SFTPA2 Antibody

SKU: orb1539455

Description

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, IHC-P, WB
Dilution range	IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:32000), WB (0.01 - 0.03 µg/ml)
Reactivity	Human
Application Notes	Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 30kD band observed in Human Lung lysates (calculated MW of 27.7kD according to NP_001087239.2). Recommended concentration: 0.01-0.03 ug/ml. Immunohistochemistry: 5 ug/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-HLDEELQATLHDFR, from the internal region of the protein sequence according to NP_005402.3NP_001087239.2NP_001158117.1NP_001158118.1NP_001092138.1.
Target	SFTPA1 + SFTPA2
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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, SFTPA1 + SFTPA2

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Anti-SFTPA1 / Surfactant Protein A antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

SFTPA1 antibody (0.01 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

SFTPA1 + SFTPA2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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