Simian Annexin A5 (ANXA5) ELISA Kit

SKU: orb781747

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A5(ANXA5).

Research Area

Cell Biology, Hematology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian ANXA5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian ANXA5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian ANXA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian ANXA5 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Primate
Sample Types	Serum, plasma and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.125 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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ANX5 ELISA Kit, ENX2 ELISA Kit, PP4 ELISA Kit, PAP-I ELISA Kit, CBP-I ELISA Kit, Anchorin CII ELISA Kit, Calphobindin I ELISA Kit, Endonexin II ELISA Kit, Lipocortin V ELISA Kit, Placental anticoagulant protein 4 ELISA Kit, Thromboplastin inhibitor ELISA Kit, Vascular anticoagulant-alpha ELISA Kit

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UniProt

Q4R4H7

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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