Simian Erythropoietin (EPO) ELISA Kit

SKU: orb781750

Featured

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Erythropoietin(EPO).

Research Area

Hematology, Immunology & Inflammation, Metabolism Research

Images & Validation

−

Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 3000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian EPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian EPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian EPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian EPO in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Primate
Sample Types	serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	46.88-3000 pg/mL
Sensitivity	20.25 pg/mL
Concentration	3000 pg/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

−

EP ELISA Kit, Epoetin ELISA Kit, Erythropoetin ELISA Kit, Hematopoietin ELISA Kit, Hemopoietin ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

UniProt

P07865

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols