Simian IFNGR1(Interferon Gamma Receptor 1) ELISA Kit

SKU: orb3127100

Description

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction

Images & Validation

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Item 1 of 1

Application Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian IFNGR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian IFNGR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian IFNGR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian IFNGR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Primate
Sample Types	serum, plasma, and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.135 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IFNGR1;IFN-gamma receptor 1; IFN-gamma-R1;CDw119;Interferon gamma receptor alpha-chain;CD119

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Simian IFNGR1(Interferon Gamma Receptor 1) ELISA Kit

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