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SIRT3 Antibody (C-term)
SKU: orb1930162
Description
Research Area
Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research
Images & Validation
−Item 1 of 5
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000, IHC-P - 1:100-500 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 43573 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−SIR2L3
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Quality Guarantee
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
SIRT3 Antibody (C-term) western blot analysis in KG-1 cell line lysates (35 ug/lane). This demonstrates the SIRT3 antibody detected the SIRT3 protein (arrow).
SIRT3 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the SIRT3 antibody detected the SIRT3 protein (arrow).
Western blot analysis of lysates from 293, HepG2 cell line (from left to right), using SIRT3 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
All lanes: Anti-SIRT3 Antibody (C-term) at 1:2000 dilution. Lane 1: human kidney lysate. Lane 2: human liver lysate. Lane 3: mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
UniProt
UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
SIRT3 Antibody (C-term) (orb1930162)
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