Smad4 Rabbit Polyclonal Antibody

SKU: orb526556

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Description

Smad4 Rabbit Polyclonal Antibody

Research Area

Cancer Biology

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	WB=1:500-2000
Reactivity	Human
Predicted Reactivity	Bovine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Smad4 (1-100/552aa)
Target	SMAD4
Molecular Weight	60 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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DPC4; JIP; MADH4; MYHRS; D18Wsu70e; SMAD4_HUMAN; SMAD4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4 (SMAD 4 | Smad4 | hSMAD4); SMAD4_MOUSE; Deletion target in pancreatic carcinoma 4 homolog; SMAD family member 4 (SMAD 4 | Smad4); SMAD4_RAT;

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Sample: Lane 1: Human K562 cell lysates, Lane 2: Human Hela cell lysates, Lane 3: Human Jurkat cell lysates, Lane 4: Human HL-60 Cell Lysates, Primary: Anti-Smad4 (orb526556) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kDa, Observed band size: 60 kDa.

Sample: Siha (Human) Cell Lysate at 30 ug, HL-60 (Human) Cell Lysate at 30 ug, Primary: Anti-Smad4 (orb526556) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 63 kD.

Quick Database Links

Gene Symbol

SMAD4

UniProt

Q13485

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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