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SMARCA2/BRM Recombinant Rabbit Monoclonal Antibody
SKU: orb1173002
Description
Research Area
Cancer Research, Coactivators and Corepressors, DNA and RNA Research, SWI/SNF (SWItch/Sucrose Non-Fermentable)
Images & Validation
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| Tested Applications | IF, IHC-Fr, IHC-P |
|---|---|
| Dilution Range | IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | E7F8 |
| Target | SMARCA2 |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: 293T, 3: MCF-7, 4: A549, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 181 kDa, Observed MW: 200 kDa.
Tissue: Mouse cerebrum, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173002.
Quick Database Links
Gene Symbol
SMARCA2
Documents Download
Datasheet
Product Information
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
SMARCA2/BRM Recombinant Rabbit Monoclonal Antibody (orb1173002)
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