SMCC

SKU: orb1298356

Description

SMCC is a heterobifunctional crosslinker that conjugates amines to thiols, enabling protein-protein and protein-antibody conjugation for biochemical research, including in vitro assay development and antibody-drug conjugate (ADC) studies.

Images & Validation

−

Key Properties

−

CAS Number	64987-85-5
MW	334.32
Purity	99.53% (May vary between batches)
Formula	C₁₆H₁₈N₂O₆
SMILES	O=C(ON1C(=O)CCC1=O)C1CCC(CN2C(=O)C=CC2=O)CC1
Target	ADC Linker
Solubility	10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.98 mM);DMSO:72.14 mg/mL (215.78 mM)

Bioactivity

−

In Vitro

N-Succinimidyl 4-(N-maleimidomethyl)cycl（SMCC) is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms). SMCC conjugation occurs via a maleimide group that is sulfhydryl (thiol; -SH) reactive and a NHS ester group that is amine reactive and forms stable, covalent protein crosslinks.

Cell Research

Branched PEI (25 kDa) was dissolved in PBS buffer to yield a concentration of 1 mg/mL. No-weigh Sulfo-SMCC was dissolved in Milli-Q water with a concentration of 10 mg/mL, then a certain of the prepared no-weigh SulfoSMCC solution was added into 2 mL PEI solution to incubate for 30 min at 37 C. After the PEI-SMCC polymer was formed, TAT solution was added into the polymer at the given molar ratio and incubated for 2 h at 4 C. The concentration of unreacted SMCC and unreacted TAT were removed using Zeba Spin Desalting Columns. The polymer PEI-SMCC-TAT was also characterized and analyzed through FTIR to check if each element was added to the PEI backbone.

Storage & Handling

−

Storage	-20°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

SMCC, ADC Linker, ADCLinker, ADC Linkers, Antibody-drug conjugates linkers, inhibit, N-Succinimidyl 4-(N-maleimidomethyl)cycl, Inhibitor

SM22 Alpha Rabbit Polyclonal Antibody [orb13691]

Applications:	FC, ICC, IF, IHC-Fr, IHC-P
Predicted Reactivity:	Bovine, Canine, Equine, Gallus, Porcine, Rabbit
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 50 μl, 100 μl, 200 μl

Item 1 of 8
CNN1 Antibody [orb3160489]
Applications:
ELISA, ICC, IHC, WB
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 50 μl, 100 μl
Item 1 of 6
CNN1 Antibody [orb3161122]
Applications:
ELISA, ICC, IHC, WB
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 50 μl, 100 μl
Item 1 of 7
MED4 Rabbit Polyclonal Antibody [orb402569]
Applications:
ELISA, IHC, WB
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Compound Identifiers

PubChem CID

125175 ↗

Key Properties

−

No computed properties available.

Protocol Information

Find More Protocols

Applications:	IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Mouse, Rat
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Recombinant
Conjugation:	Unconjugated

Applications:	ELISA, ICC, IHC, WB
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, IHC, WB
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

SMCC

Description

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

Similar Products

CNN1 Recombinant Rabbit Monoclonal Antibody [orb2563488]

SM22 Alpha Rabbit Polyclonal Antibody [orb13691]

CNN1 Antibody [orb3160489]

CNN1 Antibody [orb3161122]

MED4 Rabbit Polyclonal Antibody [orb402569]

Quality Guarantee

Compound Identifiers

Key Properties

Protocol Information

Available Sizes

Email

UK Office

US Office

CN Office

Distributors