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Description
Research Area
Musculoskeletal & Connective Tissue Research
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500, ELISA=1:5000-10000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Human |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant human SPARC protein (18-303/303aa) |
| Target | SPARC |
| Molecular Weight | 33 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−BM-40; OI17; ON; ONT; SPRC_HUMAN; SPARC; Basement-membrane protein 40 (BM-40); Osteonectin (ON); Secreted protein acidic and rich in cysteine;
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Measured by its binding ability in a indirect ELISA. Immobilized Human SPARC protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human SPARC Antibody, the EC50 is 21.47 ng/ml.
Measured by its binding ability in a indirect ELISA. Immobilized Human SPARC protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human SPARC Antibody, the EC50 is 72.97 ng/ml.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (orb1291522) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (orb1291522) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Mouse Cerebellum tissue lysates, Lane 2: Mouse Kidney tissue lysates, Lane 3: Mouse B16 cell lysates, Lane 4: Rat Cerebrum tissue lysates, Primary: Anti-SPARC (orb1291522) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kDa, Observed band size: 33 kDa.
Quick Database Links
Gene Symbol
SPARC
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SPARC Rabbit Polyclonal Antibody (orb1291522)
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