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STAT1 R31-Me2a Antibody
SKU: orb345720
Description
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| Tested Applications | DOT, ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:5000, WB: 1:500 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | STAT1 [Asym-dimethyl Arg] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 31 of human STAT. |
| Purity | Anti-STAT1 R31-Me2a was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human STAT1 surrounding aa31 and is specific for Me2a. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Cross-reactivity with STAT1 from other sources has not been determined. Minimal cross reactivity shown with STAT4 and STAT5 surrounding aa31. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.05% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 0.84 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-STAT1 Asym-dimethyl Arg31 antibody, rabbit anti-STAT1 R31me2a antibody, STAT-1, STAT 1, Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84
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Dot blot of Rabbit Anti-STAT1 R31-Me2a antibody. Antigen: non-modified, monomethylated and asymmetric dimethylated forms of the immunizing peptide. Load: 100, 10, or 1 picomolar as indicated. Primary antibody: STAT1 R31-Me2a antibody at 1:1000 for 90 min at RT. (Exposure time: 48 seconds).
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
STAT1 R31-Me2a Antibody (orb345720)
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