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Stat2 Antibody
SKU: orb344465
Description
Images & Validation
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| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:5,000 - 1:10,000, IHC: 1:2.5 µg/mL, WB: 1:1000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | 19G8.H2.H6 |
| Immunogen | This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y690 of mouse STAT2 protein. |
| Purity | This Protein-A purified antibody is directed against human STAT2 protein. A BLAST analysis was used to suggest cross-reactivity with STAT2 protein from human, rat (73%) and mouse (76%), sources based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−mouse anti-STAT2 antibody, Stat 2, Signal transducer and activator of transcription 2, p113
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HeLa whole cell lysate (p/n orb348668) was loaded at 1 µg. The blot was blocked with 1% BSA in TBST for 30 min at RT, then washed and incubated with Biorbyt's anti-Stat2 antibody in 3% BSA/TBST at 1:1000 overnight at 4C. After washing, blot was incubated with Biorbyt's HRP Rb a-Ms IgG (p/n orb347544) antibody in blocking buffer (p/n orb348637) for 30 minutes at RT.
Biorbyt's anti-Stat2 monoclonal antibody was used at a 2.5 µg/ml to detect Stat2 in squamous epithelium from human skin (40X) showing moderate to strong nuclear and faint to moderate cytoplasmic staining (image). Expression of Stat2 is expected to be cytoplasmic, and nuclear upon activation. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Stat2 Antibody (orb344465)
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