STAT3 Rabbit Polyclonal Antibody

SKU: orb1294257

Description

Rabbit polyclonal antibody to STAT3

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	Western Blot 1:1000-1:3000, Immunofluorescence 1:300-1:500
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / encompassing a sequence within the center region
Target	STAT3
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Phospho-STAT3 (Tyr705) Rabbit Polyclonal Antibody [orb608174]

Applications:	IF, IHC-Fr, IHC-P
Predicted Reactivity:	Bovine, Canine, Gallus, Guinea pig, Porcine, Rabbit, Rat, Sheep
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 50 μl, 100 μl, 200 μl

Item 1 of 6
STAT3 Rabbit Polyclonal Antibody [orb592766]
Applications:
IHC, WB
Predicted Reactivity:
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat, Sheep
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl

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Anti-STAT3 antibody at 1/2000 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294257 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294257 anti-STAT3 antibody (red) at 1:500 and 4°C. DAPI (blue) was used as the nuclear counter stain.

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Gene Symbol

STAT3

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Protocol Information

Western Blot (IB, immunoblot)

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