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Description
Research Area
Cancer Biology
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:32,000, WB: 1:1000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | STAT6 phospho Y641 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to residues surrounding Tyrosine 641 of human STAT6 protein. |
| Target | STAT6 |
| Purity | This affinity purified antibody is directed against human STAT6 pY641 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with STAT6 pY641 protein from human at 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.13 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-STAT6 pY641 antibody, phosphorylated STAT6 antibody, STAT-6, STAT 6, Signal transducer and activator of transcription 6 antibody, IL-4 Stat, Interleukin-4 Stat
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Immunohistochemistry of Rabbit anti STAT6 pY641 Antibody. Tissue: Human breast carcinoma pH9 (left) with negative control (right).
Western Blot of Rabbit anti-STAT6 pY641 antibody. Lane 1: unstimulated Hela cell lysate (p/n orb348668). Lane 2: IL-4 stimulated Hela cell lysate. Load: 10 µg per lane. Primary antibody: STAT6 pY641 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP goat anti-rabbit (orb347654) at 1:40000 for 30 min at RT. Block: 5% Blotto (p/n orb348624), for 30 min at RT. Predicted/Observed size: 94 kDa, 90 kDa. Other band(s): unspecific bands.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001171549.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
STAT6 Antibody (orb345704)
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