Sulfanilamide

SKU: orb1310915

Description

Sulfanilamide (UK-124) is a competitive inhibitor of the bacterial enzyme dihydropteroate synthetase, exhibiting an IC50 of 320 µM. This classic antibacterial agent is widely used in biochemical research to study folate biosynthesis inhibition in both in vitro enzymatic assays and in vivo bacterial growth studies.

Research Area

Cell Biology, Infectious Disease & Virology

Images & Validation

−

Key Properties

−

CAS Number	63-74-1
MW	172.20
Purity	99.94% (May vary between batches)
Formula	C₆H₈N₂O₂S
SMILES	Nc1ccc(cc1)S(N)(=O)=O
Target	Antibacterial,Autophagy,Antibiotic
Solubility	H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:14 mg/mL (81.3 mM);DMSO:50 mg/mL (290.36 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (11.61 mM)

Bioactivity

−

Target IC50	DHPS:320 μM
In Vivo	Sulfanilamide was able to inhibit a recombinant yeast strain with a wild-type FOL1 gene with an IC50 of 286.8 μg/mL, but a single mutation in the active region of the fungal dihydropyrimidine synthase from 55Trp to 55Ala or 57Pro to 57Ser resulted in resistance to Sulfanilamide with an IC50 >800 μg/mL. /The sulfonamide group of Sulfanilamide inhibits dihydropyridine synthase purified from E. coli. p-Aminobenzoic acid synthesis is used for essential folic acid in the synthesis of purines, pyrimidines, and other amino acids. The IC50 of Sulfanilamide was 320 μM for dihydropyridine synthase, and the Km was 2.5 uM for PABA. Sulfanilamide was able to inhibit the growth of Plasmodium falciparum-containing pKOS-pfPPPK-DHPS(His) bacterial cells to a certain extent with an IC50 of 380 uM. Read more...
In Vitro	Sulfanilamide was able to inhibit a recombinant yeast strain with a wild-type FOL1 gene with an IC50 of 286.8 μg/mL, but a single mutation in the active region of the fungal dihydropyrimidine synthase from 55Trp to 55Ala or 57Pro to 57Ser resulted in resistance to Sulfanilamide with an IC50 >800 μg/mL. /The sulfonamide group of Sulfanilamide inhibits dihydropyridine synthase purified from E. coli. p-Aminobenzoic acid synthesis is used for essential folic acid in the synthesis of purines, pyrimidines, and other amino acids. The IC50 of Sulfanilamide was 320 μM for dihydropyridine synthase, and the Km was 2.5 uM for PABA. Sulfanilamide was able to inhibit the growth of Plasmodium falciparum-containing pKOS-pfPPPK-DHPS(His) bacterial cells to a certain extent with an IC50 of 380 uM. Read more...

Storage & Handling

−

Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

UK124, UK-124, UK 124, Sulfanilamide, Sulphanilamide, DHPS, Antibiotic, Bacterial, inhibit, Inhibitor

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Compound Identifiers

PubChem CID

5333 ↗

Key Properties

−

No computed properties available.

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols

Sulfanilamide (orb1310915)

Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

No reviews yet

Applications:	ELISA
Reactivity:	All
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	HRP

Applications:	ELISA
Reactivity:	All
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA
Reactivity:	All
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Biotin

Sulfanilamide

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

Similar Products

Sulfadiazine Rabbit Polyclonal Antibody (HRP) [orb476362]

Sulfadiazine [orb1515758]

Sulfadiazine/BSA [orb1517053]

Sulfadiazine Rabbit Polyclonal Antibody [orb221895]

Sulfadiazine Rabbit Polyclonal Antibody (Biotin) [orb454527]

Quality Guarantee

Compound Identifiers

Key Properties

Documents Download

Request a Document

Protocol Information

Sulfanilamide (orb1310915)

Step 1: Enter information below

Step 2: Enter the in vivo formulation

Available Sizes

Email

UK Office

US Office

CN Office

Distributors