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Swine IgG (H&L) Antibody Fluorescein Conjugated
SKU: orb347965
Description
Images & Validation
−Item 1 of 2
| Tested Applications | DOT, FC, FLISA, IF, WB |
|---|---|
| Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
| Reactivity | Porcine |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Swine IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Swine IgG and Swine Serum. |
| Conjugation | FITC |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 2.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−goat Anti-Swine IgG fluorescein Conjugated Antibody, goat Anti-Swine IgG Antibody FITC Conjugation
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Dot Blot of Fluorescien Conjugated Goat anti-Swine Antibody. Antigen: Swine IgG. Load: 200 ng followed by a 3-fold serial dilution. Primary antibody: none. Secondary antibody: Fluorescien swine secondary antibody at 1:1000 for 60 min at RT.
Western Blot of Fluorescein conjugated Goat anti-Swine IgG antibody. Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein swine secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
DOT
Dot Blot
Swine IgG (H&L) Antibody Fluorescein Conjugated (orb347965)
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